Divya M. V.

As a part of DNA-protein interaction studies, Divya works on the model system chromatin to better understand compaction of 30nm fiber. Project aim is to standardize the nucleosome reconstitution method for understanding the dynamics of chromatin compaction using solid-state nanopores (fabricated in the lab). The reconstituted 12mers (12x-601) are used to develop poly-nucleosomes-PRC2 binding assays for detection using nanopores. Construction of a novel bead on a string model using the CRISPR-Cas9 technology on lambda-DNA is a classical bead-on-string model in association with nucleosome binding proteins studied by AFM and in-house nanopore system can decipher the higher order chromatin structure.